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Image Search Results
Journal: Science Advances
Article Title: Developmentally programmed histone H3 expression regulates cellular plasticity at the parental-to-early embryo transition
doi: 10.1126/sciadv.adh0411
Figure Lengend Snippet: ( A ) High-magnification images of pachytene nuclei, oocyte, and sperm from a young adult hermaphrodite, demonstrating HIS-72::mCherry (H3.3) and HIS-55::GFP (H3). White arrowheads indicate chromatin regions showing presence of H3 but absence of H3.3. ( B ) Immunofluorescence (confocal) of HIS-72::mCherry (red), HIS-55::GFP (green), H3K27me2/3 (blue), and H3K36me2 (magenta) in pachytene nuclei. White arrowheads indicate chromosome X identified by the presence of H3 and H3K27me2/3 but absence of H3.3 and H3K36me2. ( C ) Immunofluorescence (Airyscan) of HIS-72::mCherry (red), HIS-55::Ollas (green), and H3K27me2/3 (magenta; top) and H3K36me2 (magenta; bottom) in pachytene nuclei. White arrowheads indicate distinct chromatin domains marked by the colocalization of H3K27me2/3 or H3K36me2 to the presence of H3 or H3.3, respectively. ( D ) Colocalization analysis of Airyscan immunofluorescent micrographs of H3K27me2/3 or H3K36me2 with H3::Ollas and H3.3::mCherry. All quantifications = average ± SE. Unpaired t test; **** P ≤ 0.0001 and ** P ≤ 0.01. Scale bars, 5 μm.
Article Snippet: To quantify the colocalization of tagged histone proteins, H3::Ollas and H3.3::mCherry, with specific PTMs (H3K27me2/3 and H3K36me2) per nuclei, we used the
Techniques: Immunofluorescence